Unyango olusakhulayo lwe-autologous cell therapy usebenzisa i-platelet-rich plasma (PRP) inokudlala indima encedisayo kwizicwangciso ezahlukeneyo zonyango lwamayeza okuhlaziya.Kukho imfuno yehlabathi engafanelekanga yezicwangciso zokulungiswa kwezicubu zokunyanga izigulane ezine-musculoskeletal (MSK) kunye nezifo zomgogodla, i-osteoarthritis (OA) kunye nezifo ezingapheliyo ezinzima kunye namanxeba aphikisayo.Unyango lwe-PRP lusekelwe kwinto yokuba i-platelet growth factor (PGF) isekela ukuphulukiswa kwesilonda kunye nokulungiswa kwe-cascade (ukuvuvukala, ukwanda kunye nokulungiswa kwakhona).Inani leendlela ezahlukeneyo ze-PRP ziye zavavanywa kwizifundo zabantu, kwi-vitro kunye nezilwanyana.Nangona kunjalo, iingcebiso ze-in vitro kunye nezifundo zezilwanyana zihlala zikhokelela kwiziphumo ezahlukeneyo zeklinikhi, kuba kunzima ukuguqulela iziphumo zophando ezingezizo ezonyango kunye neengcebiso zendlela kunyango lwabantu.Kwiminyaka yakutshanje, inkqubela yenziwe ekuqondeni ingcamango yeteknoloji ye-PRP kunye nee-agent ze-biological, kwaye imiyalelo emitsha yophando kunye nezibonakaliso ezintsha ziye zacetywa.Kolu hlaziyo, siza kuxubusha inkqubela phambili yamva nje ekulungiseleleni nasekubunjweni kwe-PRP, kubandakanywa idosi yeplatelet, umsebenzi we-leukocyte kunye nokulawulwa kwe-immune ye-innate kunye ne-adaptive, i-5-hydroxytryptamine (5-HT) umphumo kunye nokunciphisa intlungu.Ukongezelela, siye saxubusha indlela ye-PRP ehambelana nokuvuvukala kunye ne-angiogenesis ngexesha lokulungiswa kwezicubu kunye nokuvuselelwa.Ekugqibeleni, siya kuphonononga iziphumo zamanye amachiza kumsebenzi we-PRP.
I-Autologous platelet-rich plasma (PRP) yinxalenye yolwelo lwegazi lwe-autologous peripheral emva konyango, kwaye i-concentration yeplatelet iphezulu kunesiseko.Unyango lwe-PRP lusetyenziselwe izibonakaliso ezahlukeneyo ngaphezu kweminyaka eyi-30, okubangela umdla omkhulu kwikhono le-PRP yokuzimela kwiyeza lokuvuselela.Igama elithi i-orthopedic biological agent lisanda kwaziswa ukunyanga izifo ze-musculoskeletal (MSK), kwaye liphumelele iziphumo ezithembisayo kwikhono lokuhlaziya i-heterogeneous bioactive PRP ye-cell mixes.Okwangoku, unyango lwe-PRP lukhetho olufanelekileyo lonyango kunye neenzuzo zeklinikhi, kwaye iziphumo ezixeliweyo zesigulana ziyakhuthaza.Nangona kunjalo, ukungahambelani kweziphumo zesigulane kunye nokuqonda okutsha kuye kwabangela imingeni ekusebenzeni kokusetyenziswa kwekliniki ye-PRP.Esinye sezizathu sinokuba inani kunye nokuhluka kwe-PRP kunye ne-PRP-uhlobo lweenkqubo kwiimarike.Ezi zixhobo zihluke ngokumalunga nomthamo wokuqokelela i-PRP kunye neskimu sokulungiselela, okubangelwa iimpawu ezikhethekileyo ze-PRP kunye ne-biological agents.Ukongezelela, ukungabikho kwemvumelwano malunga nokulungelelaniswa kweskimu sokulungiselela i-PRP kunye nengxelo epheleleyo yee-agent ze-biological kwisicelo seklinikhi kubangele iziphumo zengxelo ezingahambelaniyo.Iinzame ezininzi zenziwe ukubonakalisa kunye nokwahlula i-PRP okanye iimveliso ezithathwe ngegazi kwizicelo zokuvuselela amayeza.Ukongeza, ii-platelet derivatives, ezifana nee-lysates zeplatelet yomntu, ziye zacetywa kuphando lwe-orthopedic kunye ne-in vitro stem cell.
Enye yezimvo zokuqala kwi-PRP yapapashwa kwi-2006. Ingqwalasela ephambili yolu hlaziyo ngumsebenzi kunye nendlela yokwenza iiplatelet, umphumo we-PRP kwinqanaba ngalinye le-cascade yokuphulukisa, kunye nendima ephambili ye-platelet-derived factor factor. kwiimpawu ezahlukeneyo ze-PRP.Kwinqanaba lokuqala lophando lwe-PRP, umdla ophambili kwi-PRP okanye kwi-PRP-gel yayibubukho kunye nemisebenzi ethile yezinto ezininzi zokukhula kweplatelet (PGF).
Kweli phepha, siza kuxubusha ngokubanzi uphuhliso lwamva nje lwezakhiwo ze-PRP ezahlukeneyo kunye ne-platelet cell membrane receptors kunye nemiphumo yazo kwi-innate and adaptive immune system regulation.Ukongezelela, indima yeeseli ezizimeleyo ezinokuthi zibekho kwi-vial yonyango ye-PRP kunye nefuthe labo kwinkqubo yokuvuselela izicubu kuya kuxutyushwa ngokubanzi.Ukongezelela, inkqubela phambili yamva nje ekuqondeni i-PRP ye-biological agents, i-platelet dose, imiphumo ethile yeeseli ezimhlophe zegazi, kunye nemiphumo ye-PGF yoxinaniso kunye ne-cytokines kwimiphumo yesondlo se-mesenchymal stem cells (MSCs) iya kuchazwa, kubandakanywa i-PRP ejolise ngokuhlukileyo. indawo zeseli kunye nezicubu emva kokudluliselwa kwesignali yeseli kunye nemiphumo yeparacrine.Ngokufanayo, siya kuxubusha indlela ye-PRP ehambelana nokuvuvukala kunye ne-angiogenesis ngexesha lokulungiswa kwezicubu kunye nokuvuselelwa.Ekugqibeleni, siya kuphonononga umphumo we-analgesic we-PRP, umphumo wezinye izidakamizwa kumsebenzi we-PRP, kunye nokudibanisa kwe-PRP kunye neenkqubo zokuvuselela.
Imigaqo esisiseko yonyango lweplasma olutyebileyo kwiplatelet
Amalungiselelo e-PRP ayanda kakhulu kwaye asetyenziswa ngokubanzi kwiinkalo ezahlukeneyo zonyango.Umgaqo osisiseko wesayensi wonyango lwe-PRP kukuba i-injection ye-platelet ezigxininisiweyo kwindawo eyenzakeleyo inokuqalisa ukulungiswa kwezicubu, ukuhlanganiswa kwezicubu ezintsha ezidibeneyo kunye nokuvuselelwa kokujikeleza kwegazi ngokukhupha izinto ezininzi ezisebenzayo ze-biologically (izinto zokukhula, i-cytokines, i-lysosomes) kunye iiprotheni zokubambelela ezinoxanduva lokuqalisa i-hemostatic cascade reaction.Ukongezelela, iiprotheni ze-plasma (umz. i-fibrinogen, i-prothrombin, kunye ne-fibronectin) zikhona kwi-platelet-poor plasma components (PPPs).Ukugxilwa kwe-PRP kunokukhuthaza ukukhululwa kwe-hyperphysiological yezinto zokukhula ukuqala ukuphulukiswa kokulimala okungapheliyo kunye nokukhawulezisa inkqubo yokulungiswa kokulimala okukhulu.Kuzo zonke izigaba zenkqubo yokulungisa izicubu, izinto ezahlukeneyo zokukhula, i-cytokines kunye nabalawuli bezenzo zendawo bakhuthaza imisebenzi emininzi yeseli esisiseko ngokusebenzisa i-endocrine, i-paracrine, i-autocrine kunye neendlela ze-endocrine.Iinzuzo eziphambili ze-PRP zibandakanya ukhuseleko lwayo kunye neteknoloji yokulungiselela ngobuchule bezixhobo zorhwebo zangoku, ezingasetyenziselwa ukulungiselela i-biological agents ezinokuthi zisetyenziswe ngokubanzi.Okubaluleke kakhulu, xa kuthelekiswa ne-corticosteroids eqhelekileyo, i-PRP yimveliso yokuzimela ngaphandle kwemiphumo emibi eyaziwayo.Nangona kunjalo, akukho mmiselo ocacileyo kwifomula kunye nokubunjwa kwe-PRP ene-injection, kwaye ukubunjwa kwe-PRP kuneenguqu ezinkulu kwiiplatelet, umxholo we-white cell cell (WBC), i-red blood cell (RBC) ukungcola, kunye ne-PGF concentration.
Isigama se-PRP kunye nokuhlelwa
Kwiminyaka emininzi, ukuphuhliswa kweemveliso ze-PRP ezisetyenziselwa ukuvuselela ukulungiswa kwezicubu kunye nokuhlaziywa kwakhona kube yinkalo ebalulekileyo yophando lwe-biomaterials kunye nesayensi yeziyobisi.I-tissue yokuphulukisa i-cascade ibandakanya abathathi-nxaxheba abaninzi, kubandakanywa iiplatelet kunye nezinto zabo zokukhula kunye ne-cytokine granules, iiseli ezimhlophe zegazi, i-fibrin matrix kunye nezinye iicytokines ezininzi ze-synergistic.Kule nkqubo ye-cascade, inkqubo ye-coagulation eyinkimbinkimbi iya kwenzeka, kubandakanywa ukusebenza kweplatelet kunye ne-densification elandelayo kunye ne-α- Ukukhululwa kwezinto eziqulethwe kwiiplatelet particles, i-aggregation ye-fibrinogen (ekhutshwe yi-platelet okanye ikhululekile kwi-plasma) kwi-fibrin network, kunye nokwakheka. kwiplatelet embolism.
I-PRP "yendalo yonke" ifanisa ukuqala kokuphilisa
Ekuqaleni, ibinzana elithi “platelet-rich plasma (PRP)” lalibizwa ngokuba yiplatelet concentrate elalisetyenziswa kunyango lotofelo-gazi, yaye lisasetyenziswa nanamhlanje.Ekuqaleni, ezi mveliso ze-PRP zazisetyenziswa kuphela njenge-fibrin tissue adhesive, ngelixa iiplatelet zazisetyenziselwa kuphela ukuxhasa i-fibrin polymerization enamandla ukuphucula ukutywinwa kwezicubu, kunokuba njengento yokuphilisa.Emva koko, iteknoloji ye-PRP yenzelwe ukulinganisa ukuqaliswa kwe-cascade yokuphilisa.Emva koko, iteknoloji ye-PRP yashwankathelwa ngokukwazi kwayo ukwazisa nokukhulula izinto zokukhula kwi-microenvironment yendawo.Lo mdla wokuhanjiswa kwePGF uhlala ufihla indima ebalulekileyo yezinye iinxalenye kwezi zinto ziphuma egazini.Lo mdla uqiniswa ngakumbi ngenxa yokunqongophala kwedatha yenzululwazi, iinkolelo ezingaqondakaliyo, umdla wezorhwebo kunye nokungabikho komgangatho kunye nokuhlelwa.
I-biology ye-PRP concentrate inzima njengegazi ngokwalo, kwaye ingaba nzima ngakumbi kuneziyobisi zendabuko.Iimveliso ze-PRP zizinto eziphilayo eziphilayo.Iziphumo zesicelo se-PRP seklinikhi zixhomekeke kwiimpawu zangaphakathi, zendalo kunye neziguquguqukayo zegazi lesigulane, kubandakanywa nezinye iinxalenye zeselula ezinokuthi zibe khona kwisampuli ye-PRP kunye ne-microenvironment yendawo ye-receptor, enokuthi ibe kwimeko enzima okanye engapheliyo.
Isishwankathelo sesigama se-PRP esibhidayo kunye nenkqubo yokuhlela ecetywayo
Kwiminyaka emininzi, oogqirha, izazinzulu kunye neenkampani ziye zahlaselwa ukungaqondi kakuhle kunye neziphene zeemveliso ze-PRP kunye nemigaqo yazo eyahlukileyo.Abanye ababhali bachaza i-PRP njengeplatelet-kuphela, ngelixa abanye babonisa ukuba i-PRP iqulethe iiseli ezibomvu zegazi, iiseli zegazi ezimhlophe ezahlukeneyo, i-fibrin kunye neeprotheni ze-bioactive kunye noxinzelelo olukhulayo.Ngoko ke, ezininzi ezahlukeneyo ze-PRP ze-biological agents ziye zaziswa ekusebenzeni kweklinikhi.Kuyadanisa ukuba uncwadi ludla ngokungabi nankcazelo eneenkcukacha zezinto eziphilayo.Ukungaphumeleli kokulungiswa komgangatho wokulungiswa kwemveliso kunye nophuhliso lwenkqubo yokuhlelwa okulandelayo kwakhokelela ekusebenziseni inani elikhulu leemveliso ze-PRP ezichazwe ngamagama ahlukeneyo kunye nezifinyezo.Akumangalisi ukuba utshintsho kumalungiselelo e-PRP lukhokelela kwiziphumo zesigulane esingahambelaniyo.
U-Kingsley waqala ukusebenzisa igama elithi "platelet-rich plasma" ngo-1954. Kwiminyaka emininzi kamva, u-Ehrenfest et al.Inkqubo yokuqala yokwahlula ngokusekelwe kwizinto ezintathu eziphambili (i-platelet, i-leukocyte kunye nomxholo we-fibrin) yacetyiswa, kwaye iimveliso ezininzi ze-PRP zahlulwa zibe ngamacandelo amane: i-P-PRP, i-LR-PRP, i-fibrin ecocekileyo yeplatelet (P-PRF) kunye ne-leucocyte. i-PRF ecebileyo (L-PRF).Ezi mveliso zilungiswa ngenkqubo evaliweyo ngokuzenzekelayo okanye iprotocol yencwadana.Okwangoku, u-Everts et al.Ukubaluleka kokukhankanya iiseli ezimhlophe zegazi kumalungiselelo e-PRP kwagxininiswa.Bakwacebisa ukusetyenziswa kwesigama esifanelekileyo ukubonisa iinguqulelo ezingasebenziyo okanye ezisebenzayo zamalungiselelo e-PRP kunye nejel yeplatelet.
Delong et al.icetywayo inkqubo yokuhlela ye-PRP ebizwa ngokuba ziiplatelet, iiseli zegazi ezimhlophe ezisebenzayo (PAW) ezisekelwe kwinani elipheleleyo leeplatelet, kubandakanywa iindidi ezine zeplatelet concentrations.Ezinye iiparamitha ziquka ukusetyenziswa kwee-activators zeplatelet kunye nobukho okanye ukungabikho kweeseli ezimhlophe zegazi (okt neutrophils).UMishra et al.Kucetywa inkqubo efanayo yokuhlela.Kwiminyaka embalwa kamva, uMautner kunye noogxa bakhe bachaza inkqubo yokuhlela ecacileyo kunye neenkcukacha (PLRA).Umbhali ubonise ukuba kubalulekile ukuchaza inani elipheleleyo leplatelet, umxholo weeseli ezimhlophe zegazi (ezilungileyo okanye ezimbi), ipesenteji ye-neutrophil, i-RBC (elungileyo okanye engalunganga) kunye nokuba kusetyenziswe i-activation exogenous.Kwi-2016, uMagalon et al.Ulwahlulo lwe-DEPA olusekwe kwidosi yesitofu seplatelet, ukusebenza kakuhle kwemveliso, ukucoceka kwe-PRP efunyenweyo kunye nenkqubo yokuqalisa yapapashwa.Emva koko, uLana kunye noogxa bakhe baye bazisa inkqubo yokuhlelwa kwe-MARSPILL, egxininisa kwiiseli zegazi ze-mononuclear ze-peripheral.Kutshanje, iKomiti yoMgangatho weNzululwazi ikhuthaze ukusetyenziswa kwenkqubo yokuhlelwa kwe-International Society ye-Thrombosis kunye ne-Hemostasis, esekwe kuthotho lweengcebiso zokuvumelana ukulinganisa ukusetyenziswa kweemveliso zeplatelet kwizicelo zamayeza okuhlaziya, kubandakanywa neemveliso zeplatelet ezikhenkcezisiweyo kunye nethawed.
Ngokusekelwe kwinkqubo yokuhlela ye-PRP ecetywayo ngabasebenzi abahlukeneyo kunye nabaphandi, iinzame ezininzi ezingaphumeleli zokulinganisa imveliso, inkcazo kunye nefomula ye-PRP eya kusetyenziswa ngabagqirha inokwenza isigqibo esifanelekileyo, esinokuthi singenzeki kwiminyaka embalwa ezayo Ukongeza. , iteknoloji yeemveliso ze-PRP zeklinikhi ziyaqhubeka nokuphuhlisa, kwaye idatha yesayensi ibonisa ukuba amalungiselelo e-PRP ahlukeneyo afunekayo ukunyanga izifo ezahlukeneyo phantsi kweemeko ezithile.Ngoko ke, silindele ukuba iiparitha kunye neziguquko zemveliso efanelekileyo ye-PRP iya kuqhubeka ikhula kwixesha elizayo.
Indlela yokulungiselela i-PRP iyaqhubeka
Ngokwesigama se-PRP kunye nenkcazo yemveliso, iinkqubo ezininzi zokuhlela zikhutshwa kwiifom ze-PRP ezahlukeneyo.Ngelishwa, akukho mvumelwano kwinkqubo yokuhlela ebanzi ye-PRP okanye nayiphi na enye i-autologous yegazi kunye neemveliso zegazi.Ngokufanelekileyo, inkqubo yokuhlelwa kufuneka ihlawule ingqalelo kwiimpawu ezahlukeneyo ze-PRP, iinkcazo kunye ne-nomenclature efanelekileyo ehambelana nezigqibo zonyango lwezigulane ezinezifo ezithile.Okwangoku, izicelo ze-orthopedic zahlula i-PRP kwiindidi ezintathu: i-fibrin ecocekileyo ye-platelet-rich (P-PRF), i-leucocyte-rich PRP (LR-PRP) kunye ne-leucocyte-deficient PRP (LP-PRP).Nangona ichaneke ngakumbi kunenkcazo yemveliso ye-PRP ngokubanzi, iindidi ze-LR-PRP kunye ne-LP-PRP ngokucacileyo ayinayo nayiphi na into ecacileyo kumxholo weseli emhlophe yegazi.Ngenxa yeendlela zayo zokuzikhusela kunye nokuzikhusela, iiseli ezimhlophe zegazi ziye zayichaphazela kakhulu i-biology ye-intrinsic yezifo ezingapheliyo.Ngoko ke, i-PRP ye-biological agents equkethe iiseli ezimhlophe zegazi ezimhlophe zinokukhuthaza kakhulu ukulawulwa kwe-immune kunye nokulungiswa kwezicubu kunye nokuvuselelwa.Ngokukodwa, i-lymphocytes ininzi kwi-PRP, ivelisa i-insulin-efana nokukhula kwento kunye nokuxhasa ukulungiswa kwezicubu.
I-Monocytes kunye ne-macrophages idlala indima ephambili kwinkqubo yokulawulwa kwe-immune kunye nendlela yokulungiswa kwezicubu.Ukubaluleka kwe-neutrophils kwi-PRP akucaci.I-LP-PRP yamiselwa njengolungiselelo lokuqala lwe-PRP ngovavanyo olucwangcisiweyo ukuphumeza iziphumo zonyango olusebenzayo lwe-OA edibeneyo.Nangona kunjalo, uLana et al.Ukusetyenziswa kwe-LP-PRP kunyango lwe-OA yamadolo kuyachaswa, nto leyo ebonisa ukuba iiseli zegazi ezimhlophe ezithile zidlala indima ebalulekileyo kwinkqubo yokuvuvukala ngaphambi kokuvuselelwa kwezicubu, kuba zikhulula i-molekyuli e-pro-inflammatory and anti-inflammatory.Baye bafumanisa ukuba indibaniselwano ye-neutrophils kunye neeplatelet ezisebenzayo zineziphumo ezilungileyo ngakumbi kuneziphumo ezibi ekulungisweni kwezicubu.Baye babonisa ukuba iplastiki ye-monocytes ibalulekile kumsebenzi ongeyena ukuvuvukala kunye nokulungiswa kokulungiswa kwezicubu.
Ingxelo yeskimu sokulungiselela i-PRP kuphando lweklinikhi ayihambelani kakhulu.Uninzi lwezifundo ezipapashiweyo azizange zicebise indlela yokulungiselela i-PRP efunekayo ukuphindaphinda kwesikimu.Akukho mvumelwano ecacileyo phakathi kwezibonakaliso zonyango, ngoko kunzima ukuthelekisa iimveliso ze-PRP kunye neziphumo zonyango ezinxulumene nazo.Kwiimeko ezininzi ezixeliweyo, unyango lwe-platelet yoxinaniso luhlelwa phantsi kwegama elithi "PRP", nakwisalathisi esifanayo seklinikhi.Kwezinye iindawo zonyango (ezifana ne-OA kunye ne-tendinosis), inkqubela yenziwe ekuqondeni utshintsho lwamalungiselelo e-PRP, iindlela zokuhambisa, umsebenzi weplatelet kunye nezinye iinxalenye ze-PRP ezichaphazela ukulungiswa kwezicubu kunye nokuvuselelwa kwezicubu.Nangona kunjalo, uphando olongezelelweyo luyafuneka ukufikelela kwimvumelwano yesigama se-PRP esinxulumene ne-PRP ye-biological agents ukwenzela ukuba uphathe ngokupheleleyo kwaye ukhuselekile iipathologies kunye nezifo ezithile.
Ubume benkqubo yokuhlelwa kwe-PRP
Ukusetyenziswa kwe-autologous ye-PRP ye-biotherapy ixhatshazwa yi-heterogeneity yamalungiselelo e-PRP, ukubiza amagama angahambelaniyo kunye nomgangatho ophantsi wemigaqo esekelwe kubungqina (oko kukuthi, kukho iindlela ezininzi zokulungiselela ukuvelisa iibhotile zonyango).Kunokuqikelelwa ukuba umxholo we-PRP opheleleyo, ukucoceka kunye neempawu ze-biological ze-PRP kunye neemveliso ezinxulumene nazo ziyahluka kakhulu, kwaye zichaphazela ukusebenza kwe-biological kunye neziphumo zovavanyo lweklinikhi.Ukukhethwa kwesixhobo sokulungiselela i-PRP kwazisa inguqu yokuqala engundoqo.Kwiyeza lokuvuselela iklinikhi, oogqirha banokusebenzisa izixhobo ezimbini ezahlukeneyo zokulungiselela i-PRP kunye neendlela.Ukulungiswa kusebenzisa isahlukanisi segazi esiqhelekileyo, esisebenza kwigazi elipheleleyo eliqokelelwe ngokwalo.Le ndlela isebenzisa idrum ye-centrifuge eqhubekayo okanye iteknoloji yokwahlula idisk kunye namanyathelo anzima kunye athambileyo e-centrifuge.Uninzi lwezi zixhobo zisetyenziselwa utyando.Enye indlela kukusebenzisa ubuchwepheshe be-gravity centrifugal kunye nezixhobo.I-G-force centrifugation ephezulu isetyenziselwa ukwahlula umaleko otyheli we-ESR kwiyunithi yegazi equkethe iiplatelet kunye neeseli ezimhlophe zegazi.Ezi zixhobo zoxinaniso zincinci kunezahluli zeeseli zegazi kwaye zinokusetyenziswa ecaleni kwebhedi.Umahluko ģ – Ixesha lokunyanzeliswa kunye ne-centrifugation likhokelela kumahluko omkhulu kwisivuno, ukugxila, ukucoceka, ukusebenza, kunye nemeko esebenzayo yeeplatelet ezizimeleyo.Iindidi ezininzi zezixhobo zokulungiselela i-PRP zorhwebo zingasetyenziselwa kwicandelo lokugqibela, okubangela utshintsho olongezelelweyo kumxholo wemveliso.
Ukungabikho kwemvumelwano kwindlela yokulungiselela kunye nokuqinisekiswa kwe-PRP kuqhubeka nokukhokelela ekungahambelani konyango lwe-PRP, kwaye kukho ukungafani okukhulu ekulungiseleleni i-PRP, umgangatho wesampula kunye neziphumo zeklinikhi.Izixhobo ze-PRP zentengiso ezikhoyo ziqinisekisiwe kwaye zibhaliswe ngokweenkcukacha zomenzi wobunini, osombulula iinguqu ezahlukeneyo phakathi kwezixhobo ze-PRP ezikhoyo ngoku.
Qonda idosi yeplatelet kwi-vitro kunye ne-vivo
Umphumo wonyango we-PRP kunye nezinye iiplatelet zigxininise zivela ekukhululweni kwezinto ezahlukeneyo ezibandakanyekayo ekulungisweni kwezicubu kunye nokuvuselelwa.Emva kokusebenza kweeplatelet, iiplatelet ziya kwenza i-platelet thrombus, eya kusebenza njenge-matrix ye-extracellular yesikhashana ukukhuthaza ukwanda kweeseli kunye nokuhlukana.Ke ngoko, kufanelekile ukucinga ukuba idosi ephezulu yeplatelet iya kukhokelela ekugxininiseni okuphezulu kwendawo yezinto zeplatelet bioactive.Nangona kunjalo, ukulungelelaniswa phakathi kwedosi kunye nokugxininiswa kweeplatelet kunye nokugxininiswa kwe-platelet bioactive factor factor and drug isenokungalawuleki, kuba kukho ukungafani okukhulu kwi-baseline platelet count phakathi kwezigulane ezizimeleyo, kwaye kukho ukungafani phakathi kweendlela zokulungiselela i-PRP.Ngokufanayo, izinto ezininzi zokukhula kweplatelet ezibandakanyekayo kwindlela yokulungisa izicubu zikhona kwi-plasma ye-PRP (umzekelo, ukukhula kwesibindi kunye ne-insulin-efana ne-insulin factor 1).Ke ngoko, idosi ephezulu yeplatelet ayiyi kuchaphazela amandla okulungisa le miba yokukhula.
Uphando lwe-PRP lwe-in vitro luthandwa kakhulu kuba iiparitha ezahlukeneyo kwezi zifundo zinokulawulwa ngokuchanekileyo kwaye iziphumo zinokufumaneka ngokukhawuleza.Izifundo ezininzi zibonise ukuba iiseli ziphendula kwi-PRP ngendlela exhomekeke kwidosi.Nguyen kunye noPham babonise ukuba ugxininiso oluphezulu kakhulu lwe-GF aluzange lube luncedo kwinkqubo yokuvuselela iiseli, ezinokuthi zingasebenzi.Olunye uphando lwe-in vitro lubonise ukuba ukugxila okuphezulu kwe-PGF kunokuba nemiphumo emibi.Esinye isizathu sinokuba linani elilinganiselweyo le-cell membrane receptors.Ke ngoko, xa inqanaba lePGF liphezulu kakhulu xa lithelekiswa nezamkeli ezikhoyo, ziya kuba nefuthe elibi ekusebenzeni kweeseli.
Ukubaluleka kwedatha yoxinaniso lweplatelet kwi-vitro
Nangona uphando lwe-in vitro luneenzuzo ezininzi, lukwanazo ezinye izinto ezingeloncedo.I-In vitro, ngenxa yentsebenziswano eqhubekayo phakathi kweentlobo ezininzi zeeseli ezahlukeneyo kuyo nayiphi na izicubu ngenxa yesakhiwo sethishu kunye nezicubu zeselula, kunzima ukuphindaphinda kwi-vitro kwindawo yenkcubeko ene-dimensional ezimbini.Ubuninzi beeseli ezinokuchaphazela indlela yesignali yeseli ngokuqhelekileyo ingaphantsi kwe-1% yemeko yezicubu.Ithishu yesitya senkcubeko enemigangatho emibini ithintela iiseli ukuba zingavezwa kwi-matrix engaphandle kwe-extracellular (ECM).Ukongeza, itekhnoloji yenkcubeko eqhelekileyo iya kukhokelela ekuqokeleleni inkunkuma yeeseli kunye nokusetyenziswa ngokuqhubekayo kwezondlo.Ke ngoko, inkcubeko ye-in vitro yahlukile kuyo nayiphi na imeko ezinzileyo, ukunikezelwa kweoksijini yezicubu okanye ukutshintshiselana ngequbuliso kwenkcubeko, kunye neziphumo eziphikisanayo ziye zapapashwa, kuthelekiswa nesiphumo seklinikhi ye-PRP kunye nokufundwa kwe-in vitro yeeseli ezithile, iintlobo zezicubu kunye neplatelet. ugxininiso.Graziani nabanye.Kwafunyaniswa ukuba i-in vitro, impembelelo enkulu kwi-proliferation of osteoblasts kunye ne-fibroblasts ifunyenwe kwi-PRP yoxinzelelo lweplatelet ngamaxesha e-2.5 aphezulu kunexabiso lesiseko.Ngokwahlukileyo, idatha yeklinikhi ebonelelwe yiPaki kunye noogxa babonisa ukuba emva kokuxutywa komgogodla, inqanaba leplatelet ye-PRP kufuneka linyuswe ngamaxesha angaphezu kwama-5 kunesiseko sokuqala ukwenza iziphumo ezilungileyo.Iziphumo ezifanayo eziphikisanayo nazo zaxelwa phakathi kwedatha ye-tendon yokwandisa kwi-vitro kunye neziphumo zeklinikhi.
(Imixholo yeli nqaku ishicilelwe, kwaye asiboneleli nasiphi na isiqinisekiso esicacileyo okanye esichaziweyo sokuchaneka, ukuthembeka okanye ukugqibelela kokuqulathwe kweli nqaku, kwaye abanaxanduva ngezimvo zeli nqaku, nceda uqonde.)
Ixesha lokuposa: Mar-01-2023